proteinree blocking buffer (LI-COR)

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LI-COR proteinree blocking buffer
Proteinree Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intercept+protein/Intercept+(TBS)+Protein-Free+Blocking+Buffer/pm42106918-458-3-6
Average 98 stars, based on 89 article reviews

proteinree blocking buffer - by Bioz Stars, 2026-09

98/100 stars

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Article Title: RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry.
Article Snippet: The RNA is transferred with 3M NaCl pH 1 (with HCl) to an NC membrane for 90 minutes at 25 C. Post transfer, the membrane is rinsed in 1x PBS and dried on Whatman Paper (GE Healthcare). .. Dried membranes are rehydrated in Intercept Protein-Free Blocking Buffer, TBS (Li-Cor Biosciences), for 30 minutes at 25 C. After the blocking, the membranes are stained using streptavidin-IR800 (LiCor Biosciences) diluted 1:5,000 in Intercept blocking buffer for 30 minutes at 25 C. Excess streptavidin-IR800 was washed from the membranes using three washeswith 0.1%Tween-20 (Sigma) in 1x PBS for 3minutes each at 25 C. Themembranes were then briefly rinsed with PBS to remove the Tween-20 before scanning. .. Membranes were scanned on a Li-Cor Odyssey CLx scanner (Li-Cor Biosciences).

Article Title: Development and application of GlycanDIA workflow for glycomic analysis
Article Snippet: Post transfer, the membrane is rinsed in 1x PBS and dried on Whatman Paper (GE Healthcare, MA). .. Dried membranes are rehydrated in Intercept Protein-Free Blocking Buffer, TBS (Li-Cor Biosciences, NE), for 30 minutes at 25 °C. .. After the blocking, the membranes are stained using Streptavidin-IR800 (Li-Cor Biosciences, NE) diluted 1:5,000 in Intercept blocking buffer for 30 minutes at 25 °C.

Article Title: RNA N-glycosylation enables immune evasion and homeostatic efferocytosis.
Article Snippet: Vincent R. Graziano, Jennifer Porat, Marie Dominique Ah Kioon, Ivana Mejdrová, Alyssa J. Matz, Charlotta G. Lebedenko, Peiyuan Chai, John V. Pluvinage, Rafael Ricci-Azevedo, Andrew G. Harrison, Skylar S. Wright, Xinzheng Wang, Madison S. Strine, Penghua Wang, Michael R. Wilson, Sivapriya Kailasan Vanaja, Beiyan Zhou, Franck J. Barrat, Thomas Carell, Ryan A. Flynn2,10,11,12 ✉ & Vijay A. Rathinam1,12 ✉

Article Title: GCGR agonism requires GABAergic signaling in the medial basal hypothalamus to promote weight loss in obese mice
Article Snippet: Proteins were then transferred to nitrocellulose membranes (Midi Format, 0.2 μM Nitrocellulose (Bio-Rad, Cat# [1704159]) using the Trans-Blot® TurboTM Transfer System (Bio-Rad, Cat# [1704150]). .. Membranes were blocked in Intercept® Protein-Free Blocking Buffer (LICORbio, Cat# [927–80001]) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against UCP1 (Abcam Cat# [ab209483], 1:5,000) and Actin (MilliporeSigma Cat# [MAB1501], 1:2,500) diluted in Intercept® Antibody Diluent (LICORbio, Cat# [927–65001]) After washing with TBS-T, membranes were incubated with species-appropriate secondary antibodies—either goat anti-rabbit IgG (LICORbio Cat# [926–68071]) or goat anti-mouse IgG (LICORbio Cat# [926–32210]) at 1:20,000—for 1 h at room temperature. .. Membranes were washed with TBS-T then stored in TBS and protein bands were detected using the LI-COR® Odyssey M Imaging System and quantified using Image Studio software (version 5.2.5, LICORbio).

Article Title: GCGR agonism requires GABAergic signaling in the Medial Basal Hypothalamus to Promote Weight Loss in Obese Mice.
Article Snippet: Proteins were then transferred to 492 nitrocellulose membranes (Midi Format, 0.2 μM Nitrocellulose (Bio-Rad, Cat# 493 [1704159]) using the Trans-Blot® TurboTM Transfer System (Bio-Rad, Cat# [1704150]). .. 494 Membranes were blocked in Intercept® Protein-Free Blocking Buffer (LICORbio, Cat# 495 [927-80001]) for 1 hour at room temperature and incubated overnight at 4 °C with 496 primary antibodies against UCP1 (Abcam Cat# [ab209483], 1:5,000) and Actin 497 (MilliporeSigma Cat# [MAB1501], 1:2,500) diluted in Intercept® Antibody Diluent 498 (LICORbio, Cat# [927-65001]) 499 After washing with TBS-T, membranes were incubated with species-appropriate 500 secondary antibodies—either goat anti-rabbit IgG (LICORbio Cat# [926-68071]) or goat 501 anti-mouse IgG (LICORbio Cat# [926-32210]) at 1:20,000—for 1 hour at room 502 temperature. .. Membranes were washed with TBS-T then stored in TBS and protein 503 Jo urn al Pr -pr oo f bands were detected using the LI-COR® Odyssey M Imaging System and quantified 504 using Image Studio software (version 5.2.5, LICORbio).

Article Title: Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein.
Article Snippet: After transfer, the membrane was rinsed with 1× PBS and dried on Whatman paper (GE Healthcare). .. Dried membranes were rehydrated in Intercept Protein-Free Blocking Buffer, TBS (LI-COR Biosciences) for 30 min at 25 °C. .. After blocking, the membranes were stained using streptavidin IR800 (LI-COR Biosciences) diluted 1:5,000 in Intercept blocking buffer for 30 min at 25 °C.

Article Title: The redundant protein synthesis gene Aimp1 challenges the canonical inverse relationship between translation and autophagy.
Article Snippet: .. Cells were then permeabilized with either 0.1% Triton X-100, for 5 minutes, or 0.05 (w/v)% saponin, for 30 minutes, diluted in Intercept Protein-Free Blocking Buffer (LI-COR, 927-80001). ..

Article Title: Covalent Inhibition of the Peptidyl-Prolyl Isomerase Pin1 by Sulfopin Results in a Broad Impact on the Phosphoproteome of Human Osteosarcoma U2-OS Cells.
Article Snippet: Following electrophoresis, gels were immediately immersed in transfer buffer (25 mM Tris, 192 mM Glycine, 20% methanol, pH 8.3), and proteins were transferred to ImmobilonFL PVDF membranes (Millipore, #IPFL00010) using a Mini Trans-Blot Cell (BioRad) wet transfer apparatus following standard conditions. .. PVDF membranes were first blocked for 1 h at room temperature using Intercept Protein-Free Blocking Buffer (Licor, #927-80001). .. Immunoblotting was performed using primary antibodies raised against Pin1 (Rabbit, Proteintech, #10495-1-AP, 1:2000) and Vinculin (Mouse, Sigma–Aldrich, #9131, 1:1000).

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